Anti-CD14 -FITC; clone TUK4

Analysis Note

ControlPBMCs

Application

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

This Milli-Mark Anti-CD14 -FITC Antibody, clone TUK4 is validated for use in FC for the detection of CD14.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD14 is composed of 356 amino acids and is anchored to the cell membrane by linkage to glycosyl phosphatidylinositol. In addition to the 53-55 kDa cell surface form, two soluble forms have been described (Goyert, 2002). CD14 is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes (Goyert, 2002). Most tissue macrophages and Langerhans cells also express CD14 (Goyert, 1999 and 2002; Gadd, 1989). Anti-CD14, TUK4, was included in the Fourth, Fifth and Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD14 (Goyert 1995 and 1996).

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified Mouse monoclonal IgG2a conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Quality

Evaluated by flow cytometry using human PBMCs.

Specificity

Antibody recognizes Human CD14 antigen.

Storage and Stability

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

Target description

53-55 kDa (Goyert, 2002)